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HRV 3C protease (Human Rhinovirus 3C protease) is commonly used for precise removal of affinity tags from recombinant proteins under mild reaction conditions.
The applications include:
- Removal of His-tag and other fusion tags during protein purification
- Preparation of proteins for functional and biochemical assays
- Production of tag-free proteins for structural studies (e.g., X-ray crystallography, NMR)
- Generation of proteins suitable for downstream formulation or development studies